Susceptibility testing in Aspergillus species complex
نویسندگان
چکیده
منابع مشابه
In vitro susceptibility-testing in Aspergillus species.
Aspergillus species are the most common causes of invasive mould infections in immunocompromised patients. The introduction of new antifungal agents and recent reports of resistance emerging during treatment of Aspergillus infections have highlighted the need for in vitro susceptibility-testing. Various testing procedures have been proposed, including macrodilution and microdilution, agar diffu...
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Antifungal susceptibility testing of filamentous fungi has become more important given the recognition of drug-resistant organisms and the availability of therapies other than amphotericin B (AMB). As current microdilution and E-test methods are limited by a 2 to 3 day incubation time required to obtain results, a more rapid method for susceptibility testing of fungi is needed. We report here a...
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A colorimetric assay for antifungal susceptibility testing of Aspergillus species (Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus nidulans, and Aspergillus ustus) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) in the presence of menadione as an electron-coupling agent....
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متن کاملRapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.
A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by a reference method.
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2014
ISSN: 1198-743X
DOI: 10.1111/1469-0691.12514